Used in neuroscience research to quantify levels of brain-derived neurotrophic factor (BDNF) in rat brain tissue or biological fluids such as cerebrospinal fluid, serum, or plasma. This ELISA kit enables researchers to study BDNF expression in experimental models of neurodegenerative diseases, including Alzheimer's and Parkinson's disease, as well as in depression, anxiety, and other psychiatric disorders. It is also applied in studies assessing the effects of exercise, stress, drugs, or therapeutic interventions on neurotrophic support and neuronal plasticity. The assay provides a sensitive and specific means to monitor changes in BDNF during development, aging, and in response to injury or disease.