Used in biomedical research to quantify soluble RANKL levels in biological samples such as serum, plasma, or cell culture supernatants. Helps in studying bone metabolism disorders including osteoporosis, rheumatoid arthritis, and periodontal disease. Enables assessment of RANKL involvement in immune regulation and tumor-induced bone destruction. Supports drug development and evaluation of therapeutic agents targeting the RANKL/RANK/OPG pathway. Provides a reliable, sensitive, and specific method for high-throughput screening in clinical and preclinical studies.