Used in biomedical research to detect and quantify nuclear factor κB subunit p65 (NF-κBp65) in cell or tissue samples. Helps assess NF-κB signaling pathway activation, which plays a key role in inflammation, immune response, and cell survival. Commonly applied in studies of inflammatory diseases, cancer, and drug development to evaluate the effect of compounds on NF-κB activity. The kit enables high-throughput analysis in microplate format, providing a reliable and sensitive method for monitoring p65 translocation and expression levels in experimental models.