Used in biomedical research to detect and quantify nuclear factor-κB (NF-κB) activity in rat cell or tissue samples. This kit is essential for studying inflammatory responses, immune regulation, cell survival, and apoptosis, as NF-κB plays a central role in these processes. Researchers use it to evaluate the effects of drugs, toxins, or genetic modifications on NF-κB signaling pathways. Common applications include studies on neurodegenerative diseases, cancer, cardiovascular conditions, and autoimmune disorders in rat models. The assay enables high-throughput analysis with high sensitivity and specificity, making it valuable in preclinical research and drug development.