Used in bone metabolism studies to measure osteocalcin levels in rat serum or plasma, aiding research on bone formation and turnover. Commonly applied in preclinical studies related to osteoporosis, fracture healing, and the effects of drugs or hormones on bone health. Enables quantitative assessment of osteocalcin, a marker of osteoblast activity, supporting investigations in endocrinology, orthopedics, and metabolic bone disease research.