Used in biomedical research to quantify Big Endothelin levels in biological samples such as plasma or serum. Helps assess endothelial function and vascular health in experimental models. Commonly applied in studies related to hypertension, cardiovascular diseases, and pulmonary disorders. Enables researchers to monitor changes in endothelin system activity in response to drugs or disease conditions. Provides a reliable, sensitive, and specific method for high-throughput screening in preclinical research.