Used in biomedical research to detect and quantify levels of Connexin 43 (CX43) protein in mouse tissue samples, particularly in studies related to cell-to-cell communication, cardiac function, and nervous system disorders. The kit enables researchers to assess CX43 expression in conditions such as myocardial infarction, arrhythmias, neuroinflammation, and brain injury. It is also applied in developmental biology to investigate gap junction formation and tissue coordination during embryogenesis. The assay supports high-throughput analysis in preclinical studies evaluating therapeutic interventions targeting intercellular signaling pathways.