Used in immunological research to quantify levels of complement fragment C5a in biological samples such as serum, plasma, or cell culture supernatants. This ELISA kit enables researchers to study the activation of the complement system, which plays a key role in inflammation, immune response, and various disease conditions including sepsis, autoimmune disorders, and ischemia-reperfusion injury. By measuring C5a concentration, scientists can assess the extent of complement activation in experimental models, evaluate the effectiveness of therapeutic agents targeting the complement pathway, and investigate the role of C5a in disease progression. The assay is designed for high sensitivity and specificity, making it suitable for preclinical research and biomarker studies.