Used in immunological research to quantify C5a, a potent anaphylatoxin generated during complement system activation. The kit enables measurement of C5a levels in biological samples such as serum, plasma, or tissue culture supernatants. It is particularly useful in studies related to inflammation, immune response, and diseases involving complement dysregulation, including sepsis, autoimmune disorders, and ischemia-reperfusion injury. The assay provides a sensitive and specific means to monitor C5a production in experimental models, supporting evaluation of therapeutic interventions targeting the complement pathway.