Used in immunological research to quantify soluble CD27 levels in biological samples such as serum, plasma, and cell culture supernatants. Helps assess immune system activation, particularly T-cell and B-cell activity, in studies related to autoimmune diseases, infectious diseases, and cancer. Commonly applied in clinical research to monitor disease progression and response to therapy. Provides a reliable, sensitive, and specific method for detecting sCD27, supporting biomarker discovery and validation in human health and disease.