Used in biomedical research to quantify collagen cross-links in bone samples, aiding studies on bone metabolism and turnover. Helps assess bone health in conditions like osteoporosis, Paget's disease, and metabolic bone disorders. Commonly applied in clinical research and drug development to monitor the effectiveness of therapies targeting bone resorption. Enables high-throughput analysis of cross-linking biomarkers such as pyridinoline and deoxypyridinoline in serum, urine, or tissue extracts.