Used in neuroscience and biomedical research to quantify choline acetyltransferase (ChAT) levels in tissue homogenates, cell lysates, and cerebrospinal fluid. This assay helps assess cholinergic neuron integrity and function, making it valuable in studying neurodegenerative diseases like Alzheimer’s, where cholinergic signaling is impaired. The kit enables high-throughput screening in preclinical studies evaluating potential therapeutics aimed at preserving or enhancing cholinergic transmission. It is also applied in toxicology research to investigate neurotoxic effects on acetylcholine synthesis pathways.