Used in research settings to measure cortisol levels in biological samples such as saliva, serum, plasma, or urine. Helps in studying stress response, adrenal function, and hypothalamic-pituitary-adrenal (HPA) axis activity. Commonly applied in clinical research, neuroscience, psychology, and endocrinology to assess physiological effects of stress, sleep disorders, depression, and metabolic conditions. Provides a sensitive and reliable method for high-throughput screening in laboratory environments. Not intended for diagnostic use.