Used in immunological research to measure the functional activity of the complement system in rat serum. The kit enables quantification of total complement activity through the CH50 assay, which determines the dilution of serum required to lyse 50% of sensitized red blood cells. This is particularly useful in studies involving inflammation, autoimmune diseases, and immune response evaluation in preclinical animal models. Researchers use it to assess complement pathway integrity, screen therapeutic compounds affecting immune function, and monitor disease progression in experimental conditions such as sepsis, ischemia-reperfusion injury, or systemic lupus erythematosus. The ELISA format allows high-throughput, sensitive, and reproducible results without requiring live cell cultures.