Used in biomedical research to quantify Claudin-2 protein levels in cell lysates, tissue extracts, and biological fluids. Enables assessment of tight junction integrity in epithelial and endothelial cells, particularly in studies related to intestinal permeability, inflammatory bowel disease, and liver dysfunction. Supports investigation of barrier function in drug delivery research and disease models involving epithelial leakage. Commonly applied in preclinical studies to evaluate changes in Claudin-2 expression under inflammatory, infectious, or oncogenic conditions.