Used in biomedical research to quantify rat type II collagen levels in tissue samples and biological fluids. Helps assess cartilage formation and degradation in studies related to arthritis, joint disease, and regenerative medicine. Commonly applied in preclinical studies evaluating the efficacy of anti-inflammatory or chondroprotective drugs. Enables researchers to monitor extracellular matrix changes in cell cultures, particularly in chondrocyte differentiation experiments. Provides a reliable tool for investigating osteoarthritis progression and repair mechanisms in animal models.