Used in biomedical research to quantify rat type I collagen levels in tissue samples, cell cultures, or biological fluids. Helps assess extracellular matrix formation, bone and connective tissue development, and fibrosis progression in disease models. Commonly applied in studies of wound healing, osteoporosis, liver fibrosis, and kidney disease. Enables researchers to evaluate the effectiveness of therapeutic agents targeting collagen synthesis or degradation. Suitable for high-throughput screening and mechanistic studies in preclinical research.