Used in biomedical research to detect and quantify levels of SEMA3D protein in biological samples such as serum, plasma, and cell culture supernatants. This kit supports studies related to nervous system development, axon guidance, and angiogenesis. It is also applied in cancer research, particularly in investigating tumor progression and metastasis where SEMA3D signaling pathways are involved. The assay enables high-throughput analysis with high sensitivity and specificity, making it suitable for both basic research and preclinical studies.