Used in biomedical research to quantify levels of human dihydrofolate reductase (DHFR) in cell lysates, tissue extracts, or biological fluids. Enables researchers to study DHFR expression in cancer cells, monitor response to antifolate therapies like methotrexate, and investigate drug resistance mechanisms. Commonly applied in oncology, pharmacology, and molecular biology studies where precise measurement of DHFR protein concentration is required. Supports high-throughput screening and validation of therapeutic agents targeting folate metabolism.