Used in biomedical research to measure estradiol hormone levels in rabbit serum or plasma samples. Helps in studying reproductive physiology, hormonal fluctuations during estrous cycles, and effects of endocrine-disrupting compounds. Commonly applied in veterinary research, toxicology studies, and fertility investigations. Provides a sensitive and specific method for quantifying low concentrations of estradiol, enabling accurate assessment of ovarian function and hormonal responses in experimental models.