Used in biomedical research to quantify levels of CYP2E1 enzyme in human tissue samples and biological fluids. Helps assess liver function and metabolic activity, particularly in studies related to alcohol metabolism, drug-induced liver injury, and non-alcoholic fatty liver disease (NAFLD). Widely applied in toxicology and pharmacology to monitor enzyme induction or inhibition by drugs, environmental toxins, and dietary factors. Supports research into oxidative stress mechanisms, as CYP2E1 is a major source of reactive oxygen species in hepatocytes. Enables high-throughput screening in drug development for potential metabolic interactions.