Used in biomedical research to measure endothelin levels in biological samples such as plasma, serum, or cell culture supernatants. Helps in studying cardiovascular diseases, hypertension, and endothelial dysfunction. Widely applied in preclinical studies to evaluate vascular health and the effects of therapeutic compounds on endothelin secretion. Also used in research related to pulmonary arterial hypertension, chronic kidney disease, and liver cirrhosis where endothelin signaling plays a key role. Provides a reliable, sensitive, and quantitative method for detecting endothelin concentrations in human samples.