Used in research to measure zonulin levels in biological samples, helping assess intestinal permeability and gut barrier function. Commonly applied in studies related to autoimmune diseases, inflammatory bowel disease (IBD), celiac disease, and metabolic disorders like type 2 diabetes. Enables investigation into the role of gut health in systemic conditions by quantifying zonumin as a biomarker of tight junction regulation. Widely used in preclinical and clinical research settings to evaluate therapeutic interventions targeting intestinal permeability.