Used in research settings to measure zonulin levels in biological samples from rats, this ELISA kit helps assess intestinal permeability and gut barrier function. It is commonly applied in studies related to inflammatory bowel disease, leaky gut syndrome, food sensitivities, and metabolic disorders. The kit enables quantification of zonulin, a protein involved in regulating tight junctions between epithelial cells, providing insight into gut health and immune activation in rodent models. Results from this assay support investigations into the gut-microbiome axis, drug effects on intestinal integrity, and mechanisms underlying autoimmune and gastrointestinal diseases.