Used in biomedical research to detect and quantify claudin proteins in biological samples, particularly in studies related to epithelial and endothelial barrier function. Widely applied in investigating tight junction integrity, which is crucial in understanding diseases such as cancer, inflammatory disorders, and infections. The kit enables researchers to assess changes in claudin expression levels in rat models, supporting drug development and mechanistic studies in tissue permeability and cellular adhesion. Commonly used in neuroscience, oncology, and toxicology research where blood-tissue barrier function is examined.