Used in biomedical research to quantify prostaglandin F2α levels in biological samples such as plasma, serum, or tissue homogenates. Enables researchers to study reproductive physiology, including luteolysis, parturition, and estrous cycle regulation in rat models. Commonly applied in studies related to fertility, pregnancy, and ovarian function. Also useful in pharmacological research assessing the impact of drugs on prostaglandin pathways. The ELISA format allows for high sensitivity and specificity, making it suitable for detecting low concentrations of PGF2α in small sample volumes.