Used in biomedical research to measure prostaglandin F2α levels in rat biological samples such as plasma, serum, or tissue homogenates. This assay is commonly applied in reproductive studies, as PGF2α plays a key role in luteolysis, estrous cycle regulation, and parturition in rats. Researchers use the kit to investigate hormonal changes related to fertility, ovarian function, and uterine activity. It is also utilized in pharmacological studies to assess the impact of drugs or environmental factors on prostaglandin production. The ELISA format allows for high sensitivity and specificity, enabling accurate quantification of PGF2α concentrations across multiple samples.