Used in biomedical research to quantify soluble Flt-1 (sFlt-1), a key biomarker associated with angiogenesis and vascular regulation. Widely applied in studies of preeclampsia, where elevated sFlt-1 levels correlate with disease severity, enabling early detection and monitoring. Supports research in oncology by assessing sFlt-1's role in tumor angiogenesis and response to anti-angiogenic therapies. Also utilized in cardiovascular and inflammatory disease studies to investigate endothelial dysfunction and vascular permeability. The ELISA format allows for high sensitivity and specificity in measuring sFlt-1 concentrations in serum, plasma, and cell culture supernatants.