Used in biomedical research to measure glucocorticoid hormone levels in human biological samples such as serum, plasma, or cell culture supernatants. It enables researchers to study stress responses, immune regulation, and adrenal function. Commonly applied in clinical studies, endocrinology research, and drug development to assess the activity of the hypothalamic-pituitary-adrenal (HPA) axis and evaluate the impact of treatments on glucocorticoid production. The kit provides a sensitive and specific method for quantifying glucocorticoids, supporting investigations into inflammation, metabolic disorders, and psychiatric conditions.