Used in research settings to quantify levels of glutamate decarboxylase 65 (GAD65) autoantibodies in human serum or plasma. This assay is primarily applied in studies related to autoimmune diabetes, especially type 1 diabetes, where GAD65 antibodies serve as a key biomarker. The kit supports early diagnosis, risk assessment, and monitoring of disease progression. It is also utilized in research on neurological disorders linked to anti-GAD65 antibodies, such as stiff-person syndrome and certain cerebellar ataxias. The ELISA format allows for high-throughput, sensitive, and specific detection, making it valuable in clinical research and laboratory investigations.