Used in biomedical research to quantify levels of GAPDH protein in human cell or tissue samples. Commonly applied in studies related to cellular metabolism, apoptosis, and neurodegenerative diseases. Helps researchers assess GAPDH as a potential housekeeping protein in Western blot normalization, though its expression can vary under experimental conditions. Also used in cancer research, as GAPDH plays a role in glycolysis, which is often upregulated in tumor cells. The ELISA format allows for sensitive and specific detection, making it suitable for high-throughput analysis in clinical and laboratory settings.