Used in biomedical research to quantify levels of High Mobility Group Protein 1 (HMG-1), also known as HMGB1, in mouse biological samples such as serum, plasma, tissue homogenates, and cell culture supernatants. This ELISA kit is specifically designed for detecting HMGB1 in experimental models involving inflammation, sepsis, autoimmune diseases, and cancer. It enables researchers to monitor HMGB1 release during cellular stress, necrosis, or immune activation, providing insights into disease mechanisms and therapeutic responses. The assay is widely applied in preclinical studies to evaluate the efficacy of anti-inflammatory drugs and to investigate the role of HMGB1 as a damage-associated molecular pattern (DAMP) molecule in immune signaling.