Used in research settings to quantify levels of High Mobility Group Protein 1 (HMG-1), also known as HMGB1, in biological samples such as serum, plasma, and cell culture supernatants. This ELISA kit is particularly valuable in studies related to inflammation, immune response, and cellular stress, as HMGB1 is a key mediator released during tissue damage and infection. It enables researchers to monitor HMGB1 release in models of sepsis, autoimmune diseases, cancer, and ischemia-reperfusion injury. The assay provides a sensitive and specific means to assess HMGB1 concentration, supporting investigations into therapeutic interventions targeting inflammatory pathways.