Used in biomedical research to quantify levels of heat shock protein 90 in cell lysates, tissue extracts, and biological fluids. Enables study of cellular stress responses, protein folding, and chaperone activity. Widely applied in cancer research due to HSP-90's role in stabilizing oncoproteins, allowing evaluation of HSP-90 expression under various treatments such as heat shock, oxidative stress, or anti-cancer drug exposure. Supports development and screening of HSP-90 inhibitors. Provides a reliable, sensitive, and specific method for monitoring HSP-90 in experimental models of neurodegenerative diseases, inflammation, and autoimmune disorders where protein misfolding and stress pathways are involved.