Used in biomedical research to measure levels of hypoxia inducible factor-1 (HIF-1) in cell or tissue samples. Helps scientists study cellular responses to low oxygen conditions. Commonly applied in cancer research, as HIF-1 is often upregulated in tumors due to their hypoxic environment. Enables evaluation of HIF-1 activation in diseases like ischemia, cardiovascular disorders, and inflammatory conditions. Supports drug development by assessing the impact of therapeutic agents on HIF-1 signaling pathways. Provides a reliable, sensitive method for quantifying HIF-1 in experimental models, aiding in the understanding of oxygen regulation mechanisms.