Used in biomedical research to measure levels of hypoxia inducible factor (HIF) in human biological samples such as serum, plasma, or cell lysates. This kit enables researchers to study cellular responses to low oxygen conditions, which is critical in understanding processes like tumor growth, ischemic diseases, and erythropoiesin regulation. It is widely applied in cancer research, cardiovascular studies, and drug development to evaluate HIF pathway activation and the effectiveness of therapeutic interventions targeting hypoxia-related mechanisms. The assay provides a quantitative, sensitive, and specific method for detecting HIF protein concentrations, supporting both clinical and experimental investigations.