Used in research settings to quantitatively measure levels of High Mobility Group Protein 1 (HMG-1), also known as HMGB1, in biological samples such as serum, plasma, and cell culture supernatants. This ELISA kit is particularly valuable in studies related to inflammation, immune response, and cellular stress, as HMGB1 is a key mediator in these processes. It is widely applied in research on sepsis, rheumatoid arthritis, cancer, and ischemia-reperfusion injury. The assay enables high sensitivity and specificity, allowing researchers to monitor HMGB1 release from cells, evaluate drug effects on inflammatory pathways, and explore the protein’s role as a damage-associated molecular pattern (DAMP) molecule. Not intended for diagnostic use.