Used in research settings to quantify levels of high molecular weight kininogen (HMWK) in human plasma or serum samples. This assay helps investigate the role of HMWK in inflammatory processes, coagulation pathways, and vascular permeability. Commonly applied in studies related to hereditary angioedema, sepsis, thrombosis, and other conditions involving the contact activation system. Provides a reliable, sensitive, and specific method for detecting HMWK concentration, supporting both clinical research and drug development efforts focused on the kallikrein-kinin system.