Used in biomedical research to detect and quantify levels of Heat Shock Protein 27 (HSP-27) in rat samples. This kit is particularly valuable in studies related to cellular stress, inflammation, and apoptosis. Researchers apply it in neuroscience, cardiology, and cancer research to understand disease mechanisms and evaluate therapeutic interventions. The assay helps assess HSP-27 expression changes in response to injury, heat stress, or drug treatments, providing insight into protective cellular responses.