Used in biomedical research to measure hexokinase enzyme levels in rat tissue samples, particularly in studies related to glucose metabolism. Commonly applied in diabetes, cancer, and metabolic disorder research where altered glycolytic activity is a key indicator. Enables quantification of hexokinase in organs like liver, muscle, and brain, helping assess mitochondrial function and cellular energy status. Widely used in preclinical studies to evaluate therapeutic interventions targeting metabolic pathways.