Used in research settings to quantitatively measure levels of High Mobility Group Protein 1 (HMG-1), also known as HMGB1, in rat biological samples such as serum, plasma, cell lysates, and tissue homogenates. This ELISA kit is particularly valuable in studies related to inflammation, sepsis, autoimmune diseases, and ischemia-reperfusion injury, where HMGB1 acts as a key alarmin or damage-associated molecular pattern (DAMP) molecule. It enables researchers to monitor HMGB1 release during cellular stress or immune activation, supporting investigations in neurodegenerative disorders, cancer, and chronic inflammatory conditions. The assay provides high sensitivity and specificity, allowing for accurate detection of low concentrations of HMGB1, making it a critical tool in preclinical research and biomarker validation.