Used to measure levels of heparin-binding epidermal growth factor in human biological samples such as serum, plasma, or cell culture supernatants. This assay helps in research related to cell proliferation, tissue regeneration, and cancer progression. Commonly applied in studies of cardiovascular diseases, wound healing, and tumor development due to HB-EGF's role in signaling pathways that regulate cell growth and survival. Also used in drug development to assess the impact of therapeutic agents on HB-EGF expression.