Used to measure levels of vascular endothelial growth factor (VEGF) in human biological samples such as serum, plasma, or cell culture supernatants. It is widely applied in research settings to study angiogenesis, tumor growth, and inflammatory diseases. Commonly used in cancer research to assess VEGF expression in patients before and after treatment. Also utilized in cardiovascular studies to understand endothelial function and in ophthalmology research related to retinal diseases. Provides a reliable, sensitive, and quantitative method for detecting VEGF protein concentrations.