Used in biomedical research to measure Granzyme B levels in human samples such as serum, plasma, or cell culture supernatants. It is essential for studying immune responses, particularly cytotoxic T-cell and natural killer (NK) cell activity. Commonly applied in research on autoimmune diseases, viral infections, cancer immunology, and transplant rejection. The kit enables quantitative analysis of Granzyme B, a protease released by immune cells to induce apoptosis in target cells, helping assess immune activation and cell-mediated cytotoxicity.