Used to detect and quantify levels of human interferon-alpha (IFN-α) in biological samples such as serum, plasma, or cell culture supernatants. Commonly applied in research and clinical settings to study immune responses, viral infections like hepatitis or HIV, and autoimmune diseases such as systemic lupus erythematosus. Helps monitor the activity of IFN-α in therapeutic treatments and vaccine development. Provides a reliable, sensitive, and specific measurement method through enzyme-linked immunosorbent assay (ELISA) technique.