Used in biomedical research to quantify irisin levels in mouse samples, particularly in studies related to metabolism, exercise physiology, and energy homeostasis. Enables researchers to investigate the role of irisin in metabolic diseases such as obesity, diabetes, and cardiovascular conditions. Commonly applied in preclinical studies using mouse models to evaluate how physical activity influences hormone expression and tissue metabolism. Provides a reliable, sensitive method for detecting irisin in serum, plasma, and cell culture supernatants, supporting data collection in endocrinology and molecular biology experiments.