Used in immunology research to detect and quantify pro-IL-1β levels in biological samples such as cell lysates and tissue homogenates. Enables scientists to study inflammasome activation and the early stages of inflammatory responses. Commonly applied in studies related to autoimmune diseases, infection, and chronic inflammation. Supports drug development by assessing the efficacy of anti-inflammatory compounds in preclinical models. Provides a reliable, sensitive, and specific method for monitoring intracellular cytokine production without requiring protein secretion.