Used in immunological research to quantify mouse interleukin-35 (IL-35) levels in biological samples such as serum, plasma, or cell culture supernatants. This ELISA kit enables researchers to study the role of IL-35 in immune regulation, inflammation, autoimmune diseases, and cancer. It is particularly useful in experiments involving regulatory T cells (Tregs) and B cells, where IL-35 acts as an immunosuppressive cytokine. The kit provides a reliable and sensitive method for monitoring changes in IL-35 expression under different experimental conditions, supporting preclinical studies in murine models.