Used in immunology research to quantify levels of intercellular adhesion molecule-1 (ICAM-1) secreted or expressed by cells in response to inflammatory stimuli. Helps assess immune activation, monitor inflammatory diseases such as asthma, arthritis, and multiple sclerosis, and evaluate endothelial cell activation in vascular disorders. Commonly applied in studies involving cytokine signaling, leukocyte recruitment, and drug development targeting inflammation pathways. Provides a reliable, sensitive method for measuring soluble ICAM-1 in cell culture supernatants, serum, and plasma samples.