Used in biomedical research to quantify irisin levels in biological samples such as serum, plasma, and cell culture supernatants. Enables investigation of irisin's role in metabolism, exercise physiology, and diseases like obesity, diabetes, and osteoporosis. Supports studies on the browning of white adipose tissue and energy expenditure. Provides a reliable, sensitive, and specific method for detecting irisin concentrations, aiding both academic research and drug development efforts focused on metabolic health.